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Detection of apoptosis in human tumors is a useful prognostic tool, because the apoptotic process can be blocked in many tumor types. Apoptosis can be detected in paraffin sections by a variety of methodologies including the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) technique, DNA gel electrophoresis, in situ end-labeling with the ApopTag technique, and the DNA ladder assay. All these methods detect late-stage apoptotic cells and are thus most useful for prognostic purposes. To detect early-stage apoptosis, we developed a monoclonal antibody (MoAb) that recognizes a novel apoptotic epitope present on the surface of apoptotic cells. This MoAb immunostains early-stage apoptotic cells, but not necrotic cells. The epitope is not located on the caspase-3 enzyme or on caspase-8 substrate recognition site. Thus, there are two apoptotic pathways, one of which involves caspases and an endogenous inhibitor of the caspases. The other involves the release of endogenous tumor necrosis factor-alpha (TNF-alpha), and an unidentified endogenous inhibitor of TNF-alpha. This MoAb is a useful reagent for detection of tumor cells undergoing early-stage apoptosis. For prognostic purposes, it is more useful to know when apoptosis starts than to know when it ends. However, the MoAb can be used to monitor the conversion of viable tumor cells to apoptosis. When the MoAb labels tumor cells, functional prognostic criteria are also identified, including histological types, tumor grade, vascular invasion, and tumor necrosis.BIGFAST/STYLE DIVERS



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Google Earth Engine.Q:

How to get the object in RequestContext()

I’m using the following configuration. When I get a resource X, and I want to access a method Z on it, I can do:

and then access as:

My question is, what if I want to access the object X itself? Is there any way to do it without using reflection (I can’t change the design of this project)?
My question is how to do this in RequestContext(). Every resource has multiple methods (X.A(), X.B(), X.C()) and they call each other.


You can use a custom middleware. An order of execution of middlewares is from the end to the beginning of the request pipeline.
So if you put your method on the beginning of the pipeline, you can access your resource using current items with the Request